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Objective:To observe and analyze the morphological characteristic of bone marrow and peripheral blood in patients diagnosed with de novo acute leukemia.Methods:From October 1, 2015 to December 31, 2021, 1151 patients aged 47 (26, 62) years, consisting of 602 males and 549 females with newly diagnosed acute leukemia in the Department of Hematology, Affiliated Hospital of Xuzhou Medical University, were collected to preform the morphological analysis in bone marrow and peripheral blood smears. Based on the comprehensive diagnosis results of morphology, immunology, cytogenetics, and molecular biology, comparison between acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL), AML with RUNX1-RUNXITI gene, AML with CBFβ/MYH11 gene, acute promyelocytic leukemia (APL) with PML/RARA gene, AML with NPM1 gene, the rest of the AML, Ph+ALL and Ph-ALL were performed by Chi-square test along with analysis of the differences in the ratio of wood bundle cells, pseudo-Chediak-Higashi (PCH) inclusions, cytoplasmic small particles, nuclear notches, leukemia cells with cup-like changes (cup cells); as well as the differences in the micromeganuclei, early immature granulocytes, plasma cells, high eosinophils and other accompanying cells and the distribution of "grape-like" aggregation. Finally, the morphological characteristics of acute leukemia cells, the appearance and arrangement of accompanying cells were summarized.Results:Between AML and ALL, there were statistically significant differences in cytoplasmic Auer bodies[(45.5%, 0%), χ 2=211.400, P<0.01], PCH inclusion bodies[(28.9%, 0%), χ 2=114.100, P<0.01], cytoplasmic fine particles[(20.7%, 2.9%), χ 2=53.798, P<0.01], nuclear notches[(0.7%, 6.1%), χ 2=30.906, P<0.01], and goblet cells[(4.9%, 0.3%), χ 2=13.495, P<0.01], micromegakaryus [(22.4%, 0.3%), χ 2=80.398, P<0.01], plasma cells[(87.6%, 10.6%), χ 2=604.241, P<0.01], hyperacidophils[(15.3%, 1.0%), χ 2=46.116, P<0.01] showed significant differences in the "grape-like" aggregation distribution. In AML with RUNX1-RUNXITI gene, the changes of vacuoles and PCH inclusion bodies are more obvious; in AML with CBFβ/MYH11 gene, the increase of hypereosinophils is more obvious; in APL with PML/RARA gene, the increase of woodbundle is more obvious. The morphology of nuclei chromatin, nucleolus, and vacuoles were also different among the groups. Comparison between Ph+ALL and Ph-ALL showed that Ph+ALL was more prone to develop early immature granulocytes and plasma cells (all P<0.05). Conclusion:There are significant differences between AML and ALL in the characteristics of leukemia cells, the regularity of accompanying cells, and the aggregation and distribution patterns. The subtypes of AML with specific genetic abnormalities have their own characteristics in the appearance of vacuoles, PCH inclusions, hypereosinophils, woodbundle cells, and goblet cells. Ph+ALL is more prone to present early immature granulocytes and plasma cells.
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Objective:To evaluate the feasibility and accuracy of a multi-dimensional peripheral quick contrast sensitivity function (pqCSF) measurement established based on Bayesian probability estimation algorithm.Methods:A cross-sectional study was conducted.Nineteen eyes of 12 healthy emmetropic subjects in Zhongshan Ophthalmic Center of Sun Yat-sen University from September 2017 to March 2018 were included, with an average age of (22.92±2.91) years.The average spherical power and cylindrical power were (-0.34±0.52)D and (-0.30±0.42)D, respectively, and the average uncorrected vision acuity was≥1.0.Based on the Bayesian probability algorithm, the peak contrast sensitivity γ max, the peak spatial frequency ? max, the bandwidth β and the low contrast intercept δ were used to quickly describe the contrast sensitivity function (CSF) curve of the full spatial frequency through multi-dimensional pqCSF method.The 16 peripheral visual field positions of all subjects were tested at 6°, 12°, 18° and 24° eccentricity of the superior, inferior, the temporal and nasal visual field by the pqCSF method, but the 18° eccentricity of temporal field, which was near the physiological blind spot, was excluded.The area under Log CSF (AULCSF) of different peripheral visual fields and the Log CSF of 19 spatial frequencies (distributed at equal intervals in logarithmic units) were compared.This study followed the Declaration of Helsinki, and the study protocol was approved by an Ethics Committee of Zhongshan Ophthalmic Center of Sun Yat-sen University (No.2018KYPJ017). Written informed consent was obtained from each subject prior to any examination. Results:With the increase of eccentricity in different visual fields, the AULCSF decreased gradually, and there were significant differences in AULCSF between different eccentricities (all at P<0.05). The AULCSF of the nasal and temporal visual field at 6°, 12° and 24° eccentricity was significantly larger than that of the superior and inferior visual field (all at P<0.05). As the distance from the fovea was increased, the pqCSF, the AULCSF, and the high-frequency cutoff were all decreased, and the standard deviation of AULCSF was increased gradually. Conclusions:The pqCSF method can depict a relatively complete peripheral CSF curve of a wide peripheral visual field, and reflect the function quality of the peripheral vision comprehensively and accurately.
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Objective:To assess the association between visual-related quality of life (QOL) and the severity of quick contrast sensitivity function (qCSF) defects in patients with early cataract.Methods:A cross section design was performed.Ninety-two eyes of 46 patients with early cataract were enrolled in Zhongshan Ophthalmic Center from March to June in 2017.All subjects completed the Chinese version of the National Eye Institute Visual Function Questionnaire (NEI VFQ-25). The qCSF measurements were performed monocularly and binocularly while the patients wearing full spectacle correction spectacles.Rasch analysis was performed to obtain final NEI VFQ-25 scores.Best corrected visual acuity (BCVA)(LogMAR) was measured and the eye with the better or the worse visual acuity was defined accordingly.The correlation between the Rasch calibrated QOL composite scores and the qCSF measurements of the better eye, the worse eye and binoculus, including the area under Log contrast sensitivity function (AULCSF), qCSF under cut-off frequency and 1.0, 1.5, 3.0, 6.0, 12.0, 18.0 c/d were analyzed.This study protocol was approved by the Ethic Committee of Zhongshan Ophthalmic Center.This study complied with Declaration of Helsinki.Results:The Rasch calibrated QOL composite score was 64.13±4.76.Significant negative correlations were found between composite scores of QOL and the BCVA of both the better or worse eye(the better eye: r=-0.386, P=0.008; the worse eye: r=-0.413, P=0.004). Significant positive correlations were found between the composite scores of QOL and the AULCSF of binoculus, the better and worse eye (binoculus: r=0.531, P<0.001; the better eye: r=0.524, P<0.001; the worse eye: r=0.711, P<0.001); Significant positive correlations were found between the composite scores of QOL and the qCSF under cut-off of binoculus, the better and worse eye (binoculus: r=0.504; the better eye: r=0.419; the worse eye: r=0.694, P<0.01). The composite scores of QOL was positively correlated with the qCSF under 1.5, 3.0, 6.0, 12.0 c/d of binoculus, the better and worse eye (binoculus: r=0.444, 0.544, 0.545, 0.493; all at P<0.05; the better eye: r=0.374, 0.506, 0.530, 0.431; all at P<0.05; the worse eye: r=0.513, 0.679, 0.710, 0.558; all at P<0.05). The composite scores of QOL was positively correlated with the qCSF under 1.0, 18.0 c/d of binoculus and the worse eye (1.0 c/d: r=0.296, 0.292; both at P<0.05; 18.0 c/d: r=0.386, 0.321; both at P<0.05). Conclusions:The qCSF measurements can reflect visual function of patients with early cataract at different spatial frequencies.The qCSF measurements of binoculus, the better eye and the worse eye show significant correlations with the composite scores of QOL.The QOL of early cataract patients shows a strong dependency on the worse eye.
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This paper reviews the clinical research literature of auricular plaster therapy for hypertension and related commonly seen symptoms. In clinic, auricular plaster therapy combined with orally taking Chinese and Western medicine, acupoint massage, ear point bloodletting and other auxiliary therapies can lower blood pressure and improve symptoms such as dizziness, headache, forgetfulness and insomnia. Auricular plaster therapy shows significant effect in the early treatment stage of essential hypertension; for patients with sleep disorders, auricular plaster therapy can increase sleep time and improve sleep quality without changing their normal sleep mode; for patients with depression or anxiety disorder, auricular plaster therapy shows significant effect in the short-term is, but when stop the intervention, it’s not that effective in the long term, and the antihypertensive and antidepressant effects disappear quickly. The main acupoint selection of auricular plaster therapy is relatively fixed, and the manipulation is roughly the same, which is easy to be popularized.
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Objective@#To determine the personal dose level of radiation workers in medical institutions at the municipal level and below in a city, and to provide a scientific support for strengthening the radiation protection in the city’s medical institutions.@*Methods@#Information of the successful applicants for the "Radiation Worker Permit" from 174 medical institutions at the municipal level and below was collected from October 1, 2011 to December 31, 2014. The annual effective dose was calculated based on the personal dose monitoring report, and indicators including sex, permit application time, hospital level, type of occupational radiation, length of radiation work, blood test, and micronucleated lymphocyte rate were analyzed.@*Results@#Of the 1 143 radiation worker permit applications submitted by medical institutions the municipal level and below in this city from 2011 to 2014, 1 123 provided at least one personal dose monitoring report. The annual effective dose of the radiation workers was 0-4.76 mSv (mean 0.31±0.40 mSv) , and the collective annual effective dose was 351.96 mSv. The annual effective dose was significantly different between radiation workers with different times of permit application, hospital levels, and types of occupational radiation (P<0.05) . Interventional radiology workers had the highest annual effective dose (0.63 mSv) , and annual effective dose was significantly different between interventional radiology workers with different lengths of radiation work (H=10.812, P<0.05) .@*Conclusion@#The personal radiation dose of radiation workers in medical institutions at the municipal level and below in this city is maintained at a relatively low level, suggesting that the occupational environment is relatively safe for these workers. However, more focus should be placed on clinical interventional radiology workers.
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A Raman spectrum fitting method based on Voigt function was proposed. The method can be used to fit the profile of Raman spectrum produced by unknown components in sample based on Voigt functions. In the experiment, pure methanol was added by various volume fraction ( 2. 5% -80. 0%) into three base-gasoline with different compositions. It can be applied to fit the background Raman spectrum produced by basic-gasoline comprised of unknown compositions. The remained spectrum, after deducting the fitted spectrum from the mixture spectrum, was considered as the contribution attributed only to methanol. And then a linear calibration model was built based on 4 training samples to predict the concentration of methanol. The root mean square error of prediction (RMSEP) was 1. 86% (V/V) and the correlation coefficient (R2) was 0. 987. Results show that it is an effective method to solve the problem of strong spectral overlap in mixture, and it has the advantages of high generalization and few training samples.
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Objective To investigate the detection value of plasma brain natriuretic peptide(BNP) in patients with heart failure.Methods 90 patients with heart failure were selected as subjects of this experiment.These include left ventricular failure,left ventricular diastolic failure and normal cardiac function.The same period 100 cases of healthy people were selected as control group.Compared four groups of patients with plasma BNP.Results plasma BNP of Heart failure patients was higher than the healthy group( P ＜ 0.05 ).The level of the serum BNP of failure patients with left ventricular systolic function was higher than those of other heart failure patients ( all P ＜ 0.05 ).Conclusion Patientswith heart failure plasma BNP to aid medical diagnosis,it has high clinical value.
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<p><b>OBJECTIVE</b>To explore the role of µ-opioid receptors (MOR) in the central nucleus of the amygdala (CeA) in modulating sucrose solution intake in rats.</p><p><b>METHODS</b>Sprague-Dawley rats received intra-CeA injection of MOR agonist DAMGO or saline, and then underwent two bottle choice test between sucrose solution and distilled water. After intake of sucrose solution or distilled water, activated neurons in the CeA were labeled and identified with MOR/Fos-double labeling immunohistochemistry.</p><p><b>RESULTS</b>Compared with saline injection, intra-CeA injection of DAMGO significantly increased sucrose solution intake in rats over a 3-h period. Sucrose solution intake induced significantly more c-Fos and MOR/Fos double-labeled neurons in the CeA than distilled water intake.</p><p><b>CONCLUSIONS</b>The CeA participates in modulation of sucrose intake in rats, and MOR may partly mediate this mechanism.</p>
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Animales , Masculino , Ratas , Amígdala del Cerebelo , Metabolismo , Neuronas , Metabolismo , Ratas Sprague-Dawley , Receptores Opioides mu , Metabolismo , Sacarosa , MetabolismoRESUMEN
<p><b>OBJECTIVE</b>To investigate the role of N-Methyl-D-aspartic acid (NMDA)-type glutamate receptors in the central nucleus of the amygdale (CeA) in food and water intake.</p><p><b>METHODS</b>Male Sprague-Dawley rats with stainless steel cannulae implanted unilaterally into the CeA were used. The prototypic NMDA receptor agonist NMDA, or the selective NMDA receptor antagonist D(-)-2-amino-5-phosphonopentanoic acid (D-AP-5) was microinjected into the CeA of satiated and euhydrated rats.</p><p><b>RESULTS</b>Intra-CeA injection of 8.50, 17.00, or 34.00 nmol NMDA did not alter food intake but significantly increased water intake 0-1 h after the injection (F(3,32)=3.191, P=0.037) independent of food intake. Without affecting the food intake, injection of 6.34, 12.70, or 25.40 nmol D-AP-5 into the CeA significantly decreased water intake 0-1 h after the injection (F(3,28)=3.118, P=0.042) independent of food intake.</p><p><b>CONCLUSION</b>NMDA receptors in the CeA may participate in the control of water intake rather than food intake.</p>
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Animales , Masculino , Ratas , 2-Amino-5-fosfonovalerato , Farmacología , Amígdala del Cerebelo , Ingestión de Líquidos , Ingestión de Alimentos , Agonistas de Aminoácidos Excitadores , Farmacología , Antagonistas de Aminoácidos Excitadores , Farmacología , Inyecciones Intraventriculares , N-Metilaspartato , Farmacología , Ratas Sprague-Dawley , Receptores de N-Metil-D-AspartatoRESUMEN
OBJECTIVE@#To investigate the effect of high-fat (HF) diet on the body weight and the mRNA expression of melanin concentrating hormone receptor 1 (MCHR1) and leptin receptor (OB-Rb) in the adipose tissue in rats, the two important and opposite factors in regulating the body weight.@*METHODS@#Post-weaning rats were divided into 3 groups: the NC group were fed a normal-chow diet (NC) (13% calories from fat), the HF group with a HF-diet (47% calories from fat) and the PHF group pair-fed a HF-diet (47% calories from fat). At the end of 8th week, the gained bodyweight, the plasma melanin concentrating hormone (MCH) and leptin, and the expression levels of MCHR1 and OB-Rb in the adipose tissue were measured.@*RESULTS@#Both the HF-diet and pair-fed HF-diet enhanced the body weight (P0.05).@*CONCLUSION@#Chronic intake of iso-caloric HF-diet and ad libitum HF-diet obviously results in increase in the body weight, serum leptin, and MCH concentration. Diet-induced obesity and related metabolic disorders are possibly correlated with up-regulated expression of MCHR1 and down-regulated expression of OB-Rb in the adipose tissue.
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Animales , Masculino , Ratas , Tejido Adiposo , Metabolismo , Animales Recién Nacidos , Dieta Alta en Grasa , Grasas de la Dieta , Hormonas Hipotalámicas , Sangre , Leptina , Sangre , Melaninas , Sangre , Obesidad , Metabolismo , Hormonas Hipofisarias , Sangre , ARN Mensajero , Genética , Metabolismo , Ratas Sprague-Dawley , Receptores de Leptina , Genética , Metabolismo , Receptores de Somatostatina , Genética , MetabolismoRESUMEN
This study is to investigate the pharmacological effect and mechanism of action of hydroxysafflor yellow A (HSYA) on acute lung injury (ALI). The rat ALI was induced by oleic acid and lipopolysaccharide (LPS) injection. The incidence of acidosis, PaO2 (arterial blood oxygen pressure), W/D (wet weight/dry weight) and lung index (LI) were measured. Electron microscope and optical microscope were applied to observe lung morphological changes in rat. RT-PCR was used to determine TNF-alpha and ICAM-1 mRNA level. Inhibition effect of HSYA on plasma inflammatory cytokine expression was measured by ELISA. HSYA could alleviate pulmonary edema, reduce acidosis, keep PaO2 from descending, inhibit inflammatory cell infiltration, inhibit rat lung TNF-alpha and ICAM-1 mRNA expression and plasma IL-6 and IL-1beta level elevation. HSYA is an effective ingredient to remit ALI induced by oleic acid and LPS in rat.
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<p><b>OBJECTIVE</b>To explore the imaging value of digital subtraction technique on head and neck with 64-slice spiral CTA.</p><p><b>METHODS</b>958 patients of head and neck vessels were examined with the direct method, Surestart law, low-dose tes methods, Surestart subtraction method, low-dose test subtraction method by CTA, the former three kinds are non-subtraction method, the latter two kinds are subtraction method, a comparative analysis on the five methods from the image quality, imaging time, imaging values, seek for the CTA method with optimal image quality, the shortest imaging time, the best value.</p><p><b>RESULTS</b>The image quality with the direct method (2.82 points), Surestart law (3.25 points), low-dose test method (3.33 points), Surestart subtraction method (4.10 points), low-dose test subtraction method (4.18 points), gradually changed well in order, the image quality of subtraction method is better than non-subtraction method; imaging time with direct method (40.26 minutes), Surestart law (35.06 minutes), small dose test method (33.49 minutes), Surestart subtraction method (17.52 minutes), small dose test subtraction method (15.32 minutes), gradually become shorter in order, the imaging time of subtraction method is shorter than the non-subtraction method; Evaluation on the sensitivity of aneurysms and stenosis, subtraction method is more better than the non-subtraction method, the little narrow extent and the smaller aneurysm, the subtraction method is more better than non-subtraction method.</p><p><b>CONCLUSION</b>The digital subtraction method is an ideal head and neck imaging method with 64-slice spiral CTA.</p>
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Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Angiografía de Substracción Digital , Angiografía Cerebral , Métodos , Cabeza , Diagnóstico por Imagen , Cuello , Diagnóstico por Imagen , Tomografía Computarizada Espiral , MétodosRESUMEN
Objective To assess influence factors of imaging quality with 64-slice spiral CT angiography (CTA) of renal artery. Methods A total of 305 hypertension patients underwent CTA of renal artery, and 108 of them were examined with DSA. The imaging quality was classified as Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, and the degree of stenosis wass classified as <50%, 50%-74%, 75%-99% and occlusion. The sensitivity and specificity of CTA of renal artery was analyzed taking DSA as the standard. Results The influence factors of CTA of renal artery included the delayed time set, rotation time, the units dose of contrast medium and the speed of injection, breath-holding, the patients age and vascular calcification. The sensitivity and specificity of CTA of arterial stenosis <50%, 50%-74%, 75%-99%, occlusion was 76.30% and 76.80%, 89.70% and 90.40%, 96.30% and 97.10%, 100% and 100%, respectively. Conclusion The influence factors of CTA of renal artery are the delayed time set, rotation time, the units dose of contrast medium and the speed of injection, breath-holding, the patients age and vascular calcification. CTA of renal artery can meet the clinical requirements in assessing the renal arterial stenosis.
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<p><b>OBJECTIVE</b>To investigate the effect of anti-ICAm-1/LAF-1 McAb on the cytotoxicity of tumor infiltrating lymphocytes from oral squamous cell carcinoma.</p><p><b>METHODS</b>TIL were isolated from fresh tumor tissues in 15 patients with oral squamous cell carcinoma and cultured in vitro. The expression of ICAM-1/LAF-1 in fresh and activated TIL was examined by FACS. The cytotoxicity of TIL against auto tumor cells and the level of TNF-alpha produced by TIL were compared before and after interrupted with anti-ICAM-1/LAF-1 McAb.</p><p><b>RESULTS</b>The expression of ICAM-1 and LAF-1 in the activated TIL significantly increased comparing with fresh TIL (P < 0.05). Interrupted by anti-ICAM-1 McAb, the cytotoxicity of TIL against auto tumor cell was not significantly changed. Interrupted by anti-ICAM-1 and anti-LAF-1 McAb, the level of TNF-alpha produced by TIL showed no differences comparing with the control (P < 0.05), but the cytotoxicity of TIL against auto tumor cells significantly decreased (P < 0.05).</p><p><b>CONCLUSION</b>The effect of anti-LAF-1 McAb may result from steric interference of antigen-nonspecific adhesion process.</p>
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Femenino , Humanos , Masculino , Anticuerpos Monoclonales , Farmacología , Carcinoma de Células Escamosas , Alergia e Inmunología , Patología , Células Cultivadas , Citotoxicidad Inmunológica , Molécula 1 de Adhesión Intercelular , Genética , Antígeno-1 Asociado a Función de Linfocito , Genética , Linfocitos Infiltrantes de Tumor , Alergia e Inmunología , Patología , Neoplasias de la Boca , Alergia e Inmunología , PatologíaRESUMEN
<p><b>OBJECTIVE</b>The purpose of this study was to investigate the effect of an angiogenesis inhibitor (TNP-470) on the ultra micro-structural morphological changes of GNM cell line, which was derived from human oral squamous cell carcinomas in vitro.</p><p><b>METHODS</b>The GNM cells were cultured and, the effect of TNP-470 on ultra micro-structural morphological changes of GNM cells was observed under the inverted microscope, the scanning electron microscope (SEM) and the transmission electron microscope (TEM).</p><p><b>RESULTS</b>Numerous round cells, shrinkage of cellular membrane and dead cells were observed 48 hours after 2 micrograms/ml of TNP-470 was added into the GNM cellular suspension. After 72 hours, GNM cells became shortened and, the number of microvilli of the cellular surface was observed under the SEM and TEM. A large number of GNM cells turned into necrosis, accompanying with the destruction of mitochondria and endoplasmic reticula.</p><p><b>CONCLUSION</b>TNP-470 has a strong tumor cytotoxic effect on GNM cells, which may be due to its destructibility on mitochondria and endoplasmic reticula of GNM cells. TNP-470 can alter the surface structure of GNM cell membrane, which suggests that TNP-470 may interrupt the metastasis of GNM cells.</p>
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Humanos , Inhibidores de la Angiogénesis , Farmacología , Antibióticos Antineoplásicos , Farmacología , Carcinoma de Células Escamosas , Patología , Ciclohexanos , Neoplasias Gingivales , Patología , Metástasis Linfática , Neovascularización Patológica , Sesquiterpenos , Farmacología , Células Tumorales CultivadasRESUMEN
Objective: To determine the relationship of the expression of transforming growth factor ?1(TGF ?1), TGF ?2 and TGF? receptor(T?RⅡ) with the development of squamous cell carcinoma (OSCC). Methods: 40 cases of OSCC and 20 cases of normal oral mucosa were examined with SABC immunohistochemical staining for the expression of TGF ?1, TGF ?2 and T?RⅡ. Results: Semi quantitative analysis revealed that TGF ?1, TGF ?2 and T?RⅡ were expresed in OSCC and normal oral mucosa. TGF ?1, TGF ?2 were overexpressed in OSCC compared with those in normal oral mucosa, while T?RⅡ expression were reduced. Overexpression of TGF ?1, TGF ?2 and reduced expression of T?RⅡ were associated with pathological grade, clinical stage and neck lymph node metastasis( P
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砄bjective:To investigate the variation of nm23 H1 gene structure and its expression in oral squamous cell carcinoma (OSCC) and to study the relationship between nm23 H1 expression and OSCC metastasis to neck lymph nodes.Methods: nm23 H1 gene in the samples of 30 cases of OSCC,7 of normal oral mucous tissue,human OSCC cell line TSCCa and human oral metastatic cancer cell line GNM was extracted and amplified by PCR. PCR products were investegated with SSCP analysis; nm23 m RNA level in the samples was studied by in situ hybridization.The relationship between mRNA expression and metastasis to neck lymph nodes was analyzed statistically.Results: All samples showed no change in nm23 H1 gene structure except deletion in one case of OSCC;7 out of 9 cases with metastasis to neck lymph nodes showed no hybridization signal of nm23 H1 mRNA,however,only 5 were found no nm23 H1 mRNA in 21 cases without metastasis,positive rate of nm23 H1 expression in GNM cells was lower than that in TSCCa (41.2% versus 63.1%);The expression of nm23 H1 gene showed significant negative relationship with neck lymph nodes metastasis ( P
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Objective To study the correlation between histopathological findings and X-ray appearances of ameloblastomas.Methods X-ray characteristics and histopathological features in 153 cases of ameloblastomas were analyzed retrospectively, and their relation was comparatively analyzed. Results ① On radiography,the lesions appeared as multilocular in 82 cases(53.59) and unilocular in 71 cases(46.41). ② 96 cases were classic ameloblastomas,including follicular type in 13 cases and non-follicular type in 83 cases,X-ray showed unilocular appearance in 35(36.46) and multilocular appearance in 61 cases(63.54).57 cases were unicystic ameloblastoma ,X-ray showed unilocular appearance in 36 cases(63.16) and multilocular appearance in 21 cases(36.84).There was significant diffrence statistically in the X-ray features of uniloculus and multiloculus between the two histopathological patterns(P﹤0.005). Conclusion Unicystic ameloblastomas often have unilocular radiologic appearance,classic ameloblastomas are mostly of multilocular radiologic appearance,while,the honeycomb radiologic appearances are common seen follicular type ameloblastoma.
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Objective: To detect the role of hypoxia in the regulation of vascular endothelial growth factor (VEGF) and matrix metalloproteinase 2 (MMP 2) in oral squamous cell carcinoma cell lines. Methods: Oral squamous cell carcinoma TSCCa and TNM cells were treated by hypoxia at ?=1% of oxygen for 4～12 h. VEGF and MMP 2 in the cells were quantitatively determined by enzymelinked immunosorbent assay (ELISA). The mRNA expression of VEGF and MMP 2 in the two cell lines was evaluated by semi quantitative RT PCR. Results: In both cell lines VEGF and MMP 2 were increased after 4 hour hypoxia treatment (P